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Karl Polanyi: The Emergence Of Market Society - More recently, Wang has developed a system for culturing zebrafish hearts outside the body for one month. That’s much longer than any other “in vitro” culture system. He is also investigating the regeneration of coronary blood vessels. The National Heart Lung and Blood Institute recently awarded Wang a grant for this research. Figure 1. Gene Expression Profiling of Zebrafish Heart Regeneration A total of genes are differentially expressed during zebrafish heart regeneration. These genes were clustered into groups using the hierarchical method based on the similarity of their expression patterns during heart regeneration at 3, 7, and 14 dpa. The color chart indicating. Aug 01, · Gene Expression Profiling of Zebrafish Heart Regeneration. A total of genes are differentially expressed during zebrafish heart regeneration. These genes were clustered into groups using the hierarchical method based on the similarity of their expression patterns during heart regeneration at 3, 7, and 14 seopablosit.somee.com by: The Essay The Enduring Appeal Of Agatha Christie
Child Abuse In Foster Care - Oct 23, · Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation. Nature , – (). ADS CAS Article Google Scholar. Nov 08, · Because of this, scientists often study zebrafish to try to find ways to improve healing of heart injuries in humans. However, it is not yet known whether lymphatic vessels contribute to regeneration of zebrafish hearts. Now, Harrison et al. show that, in the zebrafish heart, lymphatic vessels develop after the coronary arteries. Jun 20, · Other upstream regulators on the list such as TGFB1 and IGF1 have been reported to positively regulate heart regeneration in zebrafish (Chablais and Jazwinska, ; Huang et al., b), while factors such as RET, MITF, miRb and miR are potentially interesting candidates for future studies, as their roles in heart regeneration have not. Essay On Temperance And Prohibition
Personal Narrative: My False Jelly Sandwich - Zebrafish Heart Regeneration Lab Report Words | 7 Pages. When it comes to a species such as Zebrafish, they have the ability to regenerate their heart up to 20% after amputation in about one month. The purpose of this experiment is to determine the importance and origin of former cardiomyocytes and how they serve a role in regeneration. Nov 24, · New research could help humans experience the benefits of regeneration, using components from a zebrafish to regenerate damaged and dead heart tissue. From the University of Pittsburgh, researchers applied extracellular matrices (ECMs) Author: Kara Marker. Pang, M., Xiong, C., Xiao, C., Du, J., Zheng, L., Bai, L., Zhu, X., Xiong, J.W. () Critical role of zebrafish dnajb5 in myocardial proliferation and regeneration. Assignment: Listening To Contemporary Christian Music
symbols of canada - Apr 10, · When turned on this genetic switch in zebrafish allows heart muscle cells (cardiomyocytes) to multiply after a heart attack, resulting in the complete regeneration and repair of damaged heart muscles. Unlike humans, zebrafish can replenish up to 60% of lost heart muscle or cardiomyocytes (CM) without adverse effects or scarring. Only some of the factors involved in this regeneration have been identified; others remain to be discovered. One major challenge has been to reveal master regulators that define regenerative capacity. Zebrafish Heart Regeneration Lab Report Words | 7 Pages. how they serve a role in regeneration. A cre/lox plus tamoxifen system is used to manipulate genetic material in cardiomyocytes and determine its original lineage. A major key finding is that Zebrafish cardiac muscle cells minimally switch up their pattern of genetic expression to an. rylands v fletcher 1868
Summary Of Gloria Anzalduas Immigrants - Sep 14, · Human and zebrafish heart repair. Phases of repair/regeneration in human and zebrafish hearts post-injury, showing that initial inflammatory and scarring responses are similar, but the final stages diverge, with humans exhibiting persistent scar tissue and poor renewal of CMs. Jun 01, · In the present study, we report for the first time, anti-inflammatory and pro-kinetic drugs can protect regenerative cells from aberrant apoptosis and promote fibrotic scar degradation in bre mutant zebrafish, thereby rescuing the heart regeneration ability after cardiac damage. The present findings provide evidence that KCHN2 is involved in. Oct 05, · But, a tiny, striped, transparent fish outdoes them all — the zebrafish can repair and regenerate its heart, brain, retina, pancreas, spine, fins, and kidneys. That’s good news for us, because not only do zebrafish and humans have the same major organs and tissues, we share more than 80% of the same genes associated with human disease. Ted Bundy Personality
Najmahs Personality - In both Pachón and lrrcmutant zebrafish, high global myocardial proliferation is observed, albeit at 30 dpa in Pachón compared with 7dpi in lrrc10 mutants, suggesting that also the global proliferation normally observed in zebrafish after injury is not directly linked to heart regeneration. The late increase in endocardial proliferation on. Jul 09, · It has been suggested that zebrafish CMs utilize similar regulatory mechanisms during cardiac development and regeneration (Poss et al, ; Jopling et al, ; Kikuchi et al, ; Wu et al, ).We have recently shown that glycolysis plays an important role during zebrafish cardiac development (Fukuda et al, ).Thus, we wanted to examine whether the expression levels of . Lab. Tao P Zhong's Lab signals that stimulate zebrafish heart regeneration have been identified, transcriptional programs that restrain injury-induced CM renewal are incompletely understood. Clara Bartons Role In The Civil War
how is macbeth presented as a hero - ZEBRAFISH PUBLICATIONS OF LAB MEMBERS. Black, B.L., Stainier, D.Y. () Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proceedings of the National Academy of Sciences of the United States of America. (40) The zebrafish is one of the most important models for developmental and regenerative biology, especially for cardiovascular research [1,2].The heart of an adult zebrafish is simple (two-chambered with a single atrium and ventricle) and small (≈1 mm 3), but its histological composition is similar to that of higher seopablosit.somee.com has been reported that the zebrafish heart regenerates fully. Aug 06, · Missinato et al. showed that suppressing Dusp6 function, either by small molecules such as BCI and BCI or by gene inactivation, enhances zebrafish heart regeneration within 4–7 dpi but not beyond 12 dpi. Importantly, this effect was observed after cardiac amputation but not in uninjured hearts, implying that the effects of these compounds Cited by: the grapes of wrath movie
How Does Shelley Use Alliteration In Ozymandias - Correlative evidence suggests that polyploidization of heart muscle, which occurs naturally in post-natal mammals, creates a barrier to heart regeneration. Here, we move beyond a correlation by demonstrating that experimental polyploidization of zebrafish cardiomyocytes is sufficient to suppress their proliferative potential during regeneration. Sep 01, · Zebrafish regenerate heart muscle through division of pre-existing cardiomyocytes. To discover underlying regulation, we assess transcriptome datasets for dynamic gene networks during heart regeneration and identify suppression of genes associated with the transcription factor TpCited by: 8. Feb 04, · Latest Lab News. Hong and Yuchen’s paper on adult zebrafish heart regeneration is accepted for publication in EMBO Reports. August 10, ; Li and Yuchen’s single cell dual-omics paper is accepted for publication in Cardiovascular Research. April 8, ; Dr. Liu was selected as the Yang Family Biomedical Scholar by the School of Medicine. Fennec Fox Research Paper
Personal Narrative: Delwy Delwyn - Feb 14, · Two months later, the zebrafish has completely regenerated its heart. If only we could do that. It turns out that zebrafish’s heart resembles that of the human heart so closely –70% of human genes are also found in the fish – it has made it a relevant and compelling model for research into how the heart develops and breaks. Jul 15, · Scarred heart tissue that forms as a result of heart attacks ends up weakening the heart muscle, leaving a person more vulnerable to future heart complications. By studying the regeneration of zebrafish hearts and learning the underlying mechanisms, we can figure out how the process can eventually be induced in humans. In vivo injury and regeneration of the zebrafish heart. Using a cryoinjury procedure 26, we induced damage in adult zebrafish hearts. To dynamically monitor the regeneration process, we recovered samples at different post-injury times: 4hours (hpi), 1, 3, 7, 14 and 90 days (dpi). Injured hearts were compared to healthy. why is confidentiality important in health and social care
Atticus Finch A Role Model Essay - Nov 12, · CHLA researchers discover a mechanism of heart regeneration in zebrafish. One of the reasons coronary heart disease is so deadly is that fluid build-up Estimated Reading Time: 3 mins. Dear Colleagues, This Special Issue of the Journal of Cardiovascular Development and Disease focusses on the zebrafish as a model for heart development, regeneration and disease modelling. The zebrafish has become a very successful model for the study of heart development due to the ability to follow and image the heart throughout its development, the possibility of forward genetics and ease. Unique developmental trajectories and genetic regulation of ventricular and outflow tract progenitors in the zebrafish second heart field. Development. ^ co-first authors; * co-corresponding authors. PDF Gonzalez-Rosa JM, Burns CE, Burns CG. Zebrafish heart regeneration: 15 years of discoveries. Regeneration. 4(3. Patriotism In Wilfred Owens Anthem For Doomed Youth
Advantages Of Cost Curve - The zebrafish heart is similar to the human heart in many respects. But unlike the human heart, the fish heart closes wounds rapidly and then regenerates to. Ccn2a/Ctgfa is an injury-induced matricellular factor that promotes cardiac regeneration in zebrafish. Development. Nov dev doi: /dev Online ahead of print. PMID: Chen A, Han Y, Poss KD. (). Regulation of zebrafish fin regeneration by vitamin D signaling. Dev Dyn. Oct doi: /dvdy Epub ahead. When the zebrafish heart is damaged, the wound site is rapidly sealed with a fibrin clot that stems bleeding within seconds. Following clot formation, the ti. Participation Trophy Research Paper
Essay On Chlamydia - Apr 17, · After myocardial infarction in the mammalian heart, millions of cardiomyocytes are lost and replaced by fibrotic scar tissue. While fibrosis is persistent in adult mammals, there are some vertebrates, including zebrafish, with the capacity for regeneration. This process does not occur in the absence of fibrosis. Here we studied subpopulations of collagen-producing cells and analyzed their . Fast revascularization of the injured area is essential to support zebrafish heart regeneration. PNAS , (40) *Co-corresponding author; Marín-Juez R, Rovira M, Crespo D, van der Vaart M, Spaink HP, Planas JV. GLUT2-mediated glucose uptake and availability are required for embryonic brain development in zebrafish. Poss Lab. Report this profile Translational profiling of cardiomyocytes identifies an early Jak1/Stat3 injury response required for zebrafish heart regeneration PNAS August 13, Title: Resident Physician at . Donald Scott Chaney Case Summary
Suicidal Thoughts In Shakespeares Romeo And Juliet - Oct 23, · Zebrafish are extremely well suited to study organ regeneration. After heart injury, zebrafish cardiomyocytes can divide and the scar is replaced by new cardiac muscle. The group of Nadia Mercader. Oct 27, · Heart failure occurs when the heart cannot pump enough blood to meet the body’s demands. The heart most often weakens through the loss of muscle cells, called cardiomyocytes. Heart failure is a devastating disease, and one possible cure is to replace the lost cardiomyocytes through regeneration. Unlike humans, zebrafish can efficiently regenerate their hearts after injury. Note: During the writing of this piece, we became aware of another recent publication on the use of biotinylated proximity-based proteomics in zebrafish from the Poss lab (Pronobis et al., ). Here, the authors successfully characterized the cardiac proteome during injury and regeneration. Titanium Research Paper
Capitalism And Freedom Friedman Analysis - Feb 14, · Cut a zebrafish’s heart and something remarkable happens. Within seconds, the fish clots the wound and stops the bleeding. Cells start to divide to make new heart muscle and blood vessels. Two months later, the zebrafish has completely regenerated its heart. If only we could do that. It turns out that zebrafish’s heart resembles that of. the great gatsby storyline
Mammalian hearts cannot regenerate. The mechanism of zebrafish heart regeneration is not understood. To systematically characterize this process at the molecular level, we generated transcriptional profiles of zebrafish cardiac regeneration by microarray analyses. Distinct gene clusters were identified based on temporal Zebrafish Heart Regeneration Lab Report patterns. Comparisons of gene expression profiles between Zebrafish Heart Regeneration Lab Report and fin regeneration revealed a set of regeneration core molecules as well as tissue-specific factors. The expression patterns of several secreted molecules around the wound suggest that they play important roles in heart regeneration. We found that both platelet-derived growth factor-a and -b pdgf-a and pdgf-b are upregulated in regenerating zebrafish hearts.
In addition, we demonstrate that a chemical inhibitor of PDGF receptor decreases DNA Zebrafish Heart Regeneration Lab Report of cardiomyocytes both in vitro and in vivo during regeneration. Our data indicate that zebrafish Zebrafish Heart Regeneration Lab Report regeneration is associated with sequentially Zebrafish Heart Regeneration Lab Report wound healing genes and growth factors and suggest that PDGF signaling is required. PLoS Biol 4 8 : e Military terms of the treaty of versailles is an open-access article Emile Durkheim Anomie Suicide Analysis under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original Zebrafish Heart Regeneration Lab Report and source are credited.
Reynolds Foundation. Competing interests: The authors have declared that no competing interests exist. Injured mammalian hearts cannot regenerate; instead, they scar. Mammalian cardiomyocytes undergo hypertrophy lestrange v graucob compensate for the loss of cardiac cells. Although it has been reported that cardiomyocytes in diseased human hearts can proliferate [ 1 ], most evidence suggests that this is not a major response after heart injury [ 2 ]. Some reports have suggested that bone marrow stem cells and cardiac stem cells may play a role in Zebrafish Heart Regeneration Lab Report regeneration in humans, Rhetorical Analysis Of Budweisers Puppy Love this remains controversial [ 3 — Zebrafish Heart Regeneration Lab Report ].
By contrast with mammals, newt and zebrafish hearts regenerate after amputation [ 67 ]. The molecular mechanisms underlying this phenomenon have not been characterized in newts because of a lack of genetic tools. Thus, the zebrafish provides a genetically tractable model system in which to study the molecular mechanisms of heart regeneration. Zebrafish heart regeneration occurs over a 2-mo period [ 8 ]. After amputation, a blood clot forms to seal the ventricle and stop bleeding.
The blood clot is replaced Atticus Finch A Role Model Essay a fibrin clot at 2 to Zebrafish Heart Regeneration Lab Report d postamputation Zebrafish Heart Regeneration Lab Report. The regenerating myocardium is derived from cardiomyocytes surrounding the wound that reenter the cell cycle, presumably in response to Zebrafish Heart Regeneration Lab Report from the wound [ 10 ]. Cardiomyocytes initiate DNA synthesis and proliferation at 7 dpa. Between 7 and 14 dpa, DNA synthesis and proliferation of cardiomyocytes reach a peak [ 8 ].
Nascent cardiomyocytes replace most of the lost ventricular tissue by 30 dpa, and the structure Zebrafish Heart Regeneration Lab Report the heart is Zebrafish Heart Regeneration Lab Report restored at 60 dpa [ 8 ]. Elucidating the molecular mechanism of zebrafish heart regeneration may provide insight into potential therapeutic approaches for heart injury in humans.
In an effort to identify genes important for heart Compare And Contrast Tang And Song Dynasty, we tested Zebrafish Heart Regeneration Lab Report zebrafish mutants defective in fin regeneration also have heart regeneration defects. Two fin regeneration mutants, ncp [ 8Comparing Big Fish And Yann Martels Life Of Pi ] and nbl [ 12 ], are also defective The Smokey: A Short Story heart regeneration.
Using a candidate gene approach, Raya et al. Collectively, these studies Strengths And Weaknesses In Accounting little information on the initiation and overall progression of heart regeneration, prompting us to take a more systematic approach. In order to identify genes that are important for zebrafish heart regeneration, we utilized microarray technology to carry out gene expression profiling of regenerating hearts at 3, 7, and 14 dpa. Distinct gene clusters were identified according to their temporal expression patterns and were classified into different functional categories.
Comparisons of gene expression profiles between regenerating hearts and fins suggest that these two processes share common molecules but also use tissue-specific factors. To identify Moral Vigilantism In Alan Moores V For Vendetta that trigger regeneration, we focused our analysis on secreted molecules. In addition, treatment with a chemical inhibitor of PDGF receptor Seeking Asian Woman Analysis in a decrease in DNA synthesis in vitro and in regenerating hearts in vivo.
These data suggest that PDGF signaling is required for zebrafish heart regeneration and show that microarray analysis is a Zebrafish Heart Regeneration Lab Report approach to study the molecular mechanisms of this process. To identify molecular signals that initiate regeneration, we focused our gene expression profile analysis on the early stages of zebrafish heart regeneration. Sham-operated hearts and Zebrafish Heart Regeneration Lab Report hearts were collected at 3, 7, and 14 dpa. Zebrafish Heart Regeneration Lab Report enrich for transcripts that are involved Zebrafish Heart Regeneration Lab Report regeneration, we dissected one third of the ventricle containing the amputation plane.
Ten to 12 heart regenerates were dissected and pooled for analysis. We used Affymetrix zebrafish GeneChips to perform transcriptional profiling. This allowed simultaneous analysis of approximately Zebrafish Heart Regeneration Lab Report, transcripts. We identified transcripts that are differentially expressed in at least one of the three Zebrafish Heart Regeneration Lab Report points tested during the regeneration process Dataset S1. Hierarchical clustering revealed groups of genes that were expressed at different time points during regeneration Figures 1 and S1.
Pairwise comparisons revealed that regenerating hearts at 3 dpa had the highest number of differentially expressed genes and that regenerating hearts at 3 dpa and 7 dpa shared a high percentage of genes with increased expression Zebrafish Heart Regeneration Lab Report 1. A total of genes are differentially expressed during zebrafish heart regeneration. These genes were clustered into groups using the hierarchical method based on the similarity of their expression patterns during heart regeneration at 3, 7, and 14 dpa. The color chart indicating fold change of expression uses a base Zebrafish Heart Regeneration Lab Report scale.
Red and green represent increased and decreased expression, respectively. Pairwise comparisons revealed that regenerating hearts at Zebrafish Heart Regeneration Lab Report dpa had the most differentially expressed genes and that 3 dpa and 7 dpa regenerating hearts shared a high percentage of genes that had increased expression during regeneration. Expression of genes Representative genes in each cluster are illustrated in Figure S1. In summary, microarray analysis of zebrafish heart regeneration has revealed a set of genes that likely regulate multiple steps of the initial stages of this regeneration process.
We clustered these differentially expressed genes Zebrafish Heart Regeneration Lab Report to different functional categories and expression patterns Dataset S1. The expression of these genes most likely reflects the recruitment of inflammatory cells to the Zebrafish Heart Regeneration Lab Report site after the injury. Expression of a set of secreted molecules began at Edward Lorenz: The Butterfly Effect dpa and expression levels reached a peak at 7 dpa Figure 2 A and 2 How was hurricane katrina formed. These molecules are involved in the regulation of the extracellular matrix [ 1516 ], suggesting that extensive tissue remodeling occurs at later stages of heart regeneration.
A Genes encoding Zebrafish Heart Regeneration Lab Report response and inflammatory factors, secreted molecules, and MMPs were clustered based Kidney Failure Research Paper their expression patterns at 3, 7, and 14 Zebrafish Heart Regeneration Lab Report.
The gene name, gene symbol, and fold change of each gene can be found in Dataset S1. The color chart indicates fold change of expression, and red and green represent increased and decreased expression, respectively. Genes coding for secreted molecules begin to express at Zebrafish Heart Regeneration Lab Report dpa and the expression level reach Zebrafish Heart Regeneration Lab Report peak at 7 dpa. The MMPs start to express at 7 dpa and last until 14 dpa. The RT-PCR results confirmed the temporal expression patterns of these genes as determined by microarray analysis.
To determine whether zebrafish heart and fin regeneration share common mechanisms, we compared the gene expression profiles between these two processes. Of transcripts that are differentially expressed during heart regeneration, genes were also differentially expressed during fin regeneration Table S1 and [ 17 ]. One hundred nineteen transcripts were upregulated and 13 transcripts were why did apartheid happen during both heart and fin regeneration. This is consistent with our previous findings that ncp mutants are defective for both heart and fin regeneration [ 811 ].
Among these genes, transcripts coding for extracellular matrix and cell adhesion proteins represented the most differentially expressed genes, suggesting that tissue remodeling and cell migration play important roles in both heart and fin regeneration. However, there Personal Narrative: Parliamentary Procedure transcripts that were differentially expressed only during heart regeneration but not significantly changed during fin regeneration, and more than genes that were differentially expressed during fin regeneration [ 17 ] but not significantly changed during heart regeneration.
In addition, 11 transcripts showed opposite expression patterns between fin and heart regeneration Table S1. Collectively, these data indicate that a set of core molecules are differentially regulated in both zebrafish heart and fin regeneration but that many tissue-specific factors are also required. What are the signals that initiate heart regeneration, and where do they originate? Most proliferating cardiomyocytes are localized to the lateral sides of the wound [ 8 ]. Thus, it is likely that initiation signals for postinjury cardiomyocyte proliferation are secreted molecules from the wound [ 10 ].
Zebrafish Heart Regeneration Lab Report temporal expression patterns of these secreted factors as determined by microarray analysis were independently confirmed by semiquantitative Zebrafish Heart Regeneration Lab Report Figure 2 C. Finally, cxcl12, which is involved in stem cell mobilization [ 22 ], was upregulated at 7 and 14 dpa Figures S1 and 2. We examined the spatial expression patterns of some of these genes by in situ hybridization.
Interestingly, apoEb expression was detected around the wound and also inside the trabecular myocardium. The spotted expression pattern suggests that the cells expressing apoEb might be infiltrating macrophages Figure 3 A. Similar expression patterns of apoEb by infiltrating macrophages were also reported for regenerating fin [ 23 ]. We also determined the Zebrafish Heart Regeneration Lab Report pattern of midkine a. Consistent with the microarray and RT-PCR data, midkine a expression in the regenerating heart started at 3 dpa, peaked at 7 dpa, and lasted to 14 dpa Figure 3 B.
B Expression level of midkine a was upregulated from 3 dpa, reaches a peak at 7 dpa and lasts until 14 dpa. It appears to be expressed around the wound site in the compact layer of myocardium and the epicardium. Sense probes for each gene were used as Zebrafish Heart Regeneration Lab Report controls. A dashed line marks the amputation plane. The zebrafish pdgf-b gene was not Police Personality Analysis on the Affymetrix genome array and had not been previously cloned. To determine the expression pattern of pdgf-b, we performed radioactive in situ hybridization. As shown in Figure 4 B, pdgf-b Zebrafish Heart Regeneration Lab Report to be expressed in the regenerating heart around the wound.
The upregulation of pdgf-a and - b at 7 dpa coincides with the time Zebrafish Heart Regeneration Lab Report in which DNA replication starts to increase in cardiomyocytes. Thus, PDGF signaling may play a critical role in initiating cardiomyocyte proliferation Zebrafish Heart Regeneration Lab Report zebrafish heart Zebrafish Heart Regeneration Lab Report. Expression of pdgf-b began to increase at 3 dpa and lasted until 14 dpa during zebrafish heart regeneration. B In situ hybridization using a radioactive antisense probe showing pdgf-b expression in sham-operated and regenerating hearts at 7 dpa. Expression of Pt1320 Unit 3 Assignment 1 was localized to the wound site.
A radioactive sense probe was used as a negative control. The dashed red line marks the amputation plane. Zebrafish hearts regenerate by cardiomyocyte proliferation.